There are 20,000 items in the glass collections documenting the history of glassmaking from Antiquity to the present day, the largest section being examples of Czech origin. There is a large group of Renaissance Bohemian glass with enamel paintings and cut glass spanning from late Renaissance period to the 20th century. Glass from other glassmaking regions of Europe includes Venetian glass and Venetian style glass (a la façon de Venice), Dutch and Spanish in particular. Apart from Bohemian Baroque cut glass, there is glass produced in Silesia, Brandenburg, Thuringia, Saxony, Hessen and Lauenstein, as well as Russia.

A unique collection of Bohemian double-walled glass and glass painted with Swarzlot, the so-called Hausmalerei, which appears also on porcelain, are among the masterpieces of Baroque glass the Museum is proud of. Glass painted in the Rococo style from the second half of the 18th century was of particular importance for the development of painted glass in Bohemia. Recently two beakers with Allegories of Chastity and Wisdom were added to the remarkable collection thanks to the Society of Friends of the MDA, who bought them from the Fisher collection in Prague.

The collection of Bohemian glass in the Biedermeier style from the first half of the 19th century, along with the Art Nouveau pieces, and 20th century mass produced and one-off pieces of glass, all have a special place within the Museum holdings. The remarkable group of Czech Art Nouveau glass from Klostermühle (Lötz), Harrachov, and Karslbad (Moser) is complemented by French glass produced by leading makers, Gallé, Daum and A. Rousseau.

Tankard with the Assumption and a crest of family Schürer
Bohemia, 1647
clear glass, enamelled